Sat1 Knockout cell line (RAW264.7)

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Sat1 Knockout cell line (RAW264.7) Order

Sat1 Knockout cell line (RAW264.7)
Catalog#
YKO-M211
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Sat1 Knockout cell line (RAW264.7)
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Detailed Product Information

Product category
KO Cell Lines
cell name
Sat1 Knockout cell line (RAW264.7)
Species
Gene Name
Sat1
Gene ID
20229
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 2 (based on transcript Sat1-202)
Validation: PCR+Sanger Sequencing
Sat1 Knockout cell line (RAW264.7) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Sat1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the RAW264.7 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Sat1 gene knockout Sat1 Knockout cell line (RAW264.7) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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